G8 HPLC Analyzer


The G8 HPLC Analyzer is the next generation of Tosoh's industry leading HPLC testing systems for fast and accurate HbA1c results. Tosoh designed the G8 HPLC Analyzer from the user's perspective, enhancing overall safety and convenience of operation. The system utilizes Ion-exchange HPLC testing method, which is the gold standard for HbA1c diabetes measurement.

G8 HPLC Analyzer System Features

  • Stable A1c results in 1.6 minutes
  • Less than 2% CVs
  • Compact footprint - 21"W x 20"D x 19"H
  • Automated daily maintenance
  • 90 or 290 Sample Loader availability
  • Flexibility of multiple sample types and volumes
  • NGSP Certified

G8 HPLC Analyzer System Features

The Tosoh G8 HPLC Analyzer provides direct determination of stable HbA1c. The system is used for in vitro diagnostic measurement of HbA1c in blood specimens. The G8 uses a non-porous column and microcomputer technology to quickly and accurately measure the stable portion of HbA1c as a percentage of the total amount of hemoglobin present in the sample. The G8 provides accurate and precise separation of HbA1c from other hemoglobin fractions. Off-line pretreatment is not required, and there is no interference from the labile portion of HbA1c.

The analyzer dilutes the whole blood specimen with Hemolysis & Wash Solution and then injects a small volume of the treated specimen onto the TSKgel Glyco HSi Variant Column. Separation is achieved by utilizing differences in ionic interactions between the cation exchange group on the column resin surface and the hemoglobin components in a step gradient elution. The hemoglobin frations (designated as A1a, A1b, F, LA1c, SA1c, A0, and H-V0, H-V1, H-V2) are subsequently removed from the column material by performing a step-wise elution using Elution Buffers HSi Variant 1, 2, and 3 that have specific salt and pH concentrations.

The separated hemoglobin components pass through the LED photometer flow cell where changes in absorbance are measured at 415nm. The G8 software integrates and reduces the raw data, and then calculates the relative percentages of each hemoglobin fraction. The print-out consists of the numerical results and the chromatogram. This represents the changes in absorbance versus retention time for each peak fraction. An analysis requires only 1.6 minutes.

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